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primescript reverse transcriptase kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript reverse transcriptase kit
    Primescript Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+kit/PrimeScript+Reverse+Transcriptase/pm42883128-70-12-16
    Average 97 stars, based on 4949 article reviews
    primescript reverse transcriptase kit - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real-time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real-Time PCR System (Bio-Rad). ..

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure.
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real- time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real- Time PCR System (Bio- Rad). ..

    Article Title: Phytochemical characterization and anti-inflammatory evaluation of compounds extracted from Ficus erecta roots.
    Article Snippet: Ethnopharmacology relevance: The roots of Ficus erecta have been historically used in East Asian ethnomedicine for the treatment of various inflammatory conditions, particularly rheumatic and arthritic disorders.. In regions such as southern China, Korea, and Japan, decoctions or topical formulations derived from the root are traditionally applied to alleviate symptoms of rheumatoid arthritis, joint swelling, and chronic musculoskeletal pain.. These practices reflect longstanding empirical knowledge and suggest the presence of bioactive constituents with potential anti-inflammatory and immunomodulatory activities, warranting scientific validation and mechanistic

    Reverse Transcription:

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real-time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real-Time PCR System (Bio-Rad). ..

    Article Title: Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial Cells
    Article Snippet: .. After chloroform extraction and isopropanol precipitation, RNA was reverse-transcribed into cDNA using a reverse transcriptase kit (Cat. No. #RR036A; Takara Ltd., Otsu, Japan). .. Quantitative PCR was performed using TB Green Premix Ex Taq II (Cat. No. #RR820A; Takara Ltd). on an ABI 7300 realtime PCR system (Applied Biosystems Inc).

    Article Title: Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial Cells
    Article Snippet: .. After chloroform extraction and isopropanol precipitation, RNA was reverse-transcribed into cDNA using a reverse transcriptase kit (Cat. No. #RR036A; Takara Ltd., Otsu, Japan). .. Quantitative PCR was performed using TB Green Premix Ex Taq II (Cat. No. #RR820A; Takara Ltd). on an ABI 7300 real-time PCR system (Applied Biosystems Inc).

    Article Title: Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver disease via GLUD1/α-KG axis
    Article Snippet: Journal Pre-proofs Article Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver dis‐ ease via GLUD1/α-KG axis Mengya Geng, Fanzheng Meng, Hairui Li, Lijie Sun, Xuedan Sun, Yu Ding, Rongdongqing Shi, Zhihua Wang, Yabin Huang, Jizhou Wang, Yao Liu, Jiabei Wang, Peter J.. Little, Suowen Xu, Lianxin Liu, Jianping Weng, Sihui Luo PII: S2095-9273(26)00365-8 DOI: https://doi.org/10.1016/j.scib.2026.04.016 Reference: SCIB 3996 To appear in: Science Bulletin Received Date: 28 October 2025 Revised Date: 24 February 2026 Accepted Date: 3 April 2026 Please cite this article as: M. Geng, F. Meng, H. Li, L. Sun, X.. Sun, Y. Ding, R. Shi, Z. Wang, Y. Huang, J. Wang, Y. Liu, J. Wang, P.J.

    Article Title: Synthesis of transcripts using VSW-3 RNA polymerase
    Article Snippet: Gibson assembly kit, T4 RNA ligase II, rNTP, DNase I, Apyrase and T7 RNA polymerase (#M0251L) were from New England Biolabs. .. Reverse transcriptase kit (#6110A) and PrimeSTAR Max DNA Polymerase (#R045A) were from TaKaRa. .. RNA Clean kit was from New England Biolabs.

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure.
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real- time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real- Time PCR System (Bio- Rad). ..

    Article Title: Phytochemical characterization and anti-inflammatory evaluation of compounds extracted from Ficus erecta roots.
    Article Snippet: Ethnopharmacology relevance: The roots of Ficus erecta have been historically used in East Asian ethnomedicine for the treatment of various inflammatory conditions, particularly rheumatic and arthritic disorders.. In regions such as southern China, Korea, and Japan, decoctions or topical formulations derived from the root are traditionally applied to alleviate symptoms of rheumatoid arthritis, joint swelling, and chronic musculoskeletal pain.. These practices reflect longstanding empirical knowledge and suggest the presence of bioactive constituents with potential anti-inflammatory and immunomodulatory activities, warranting scientific validation and mechanistic

    Article Title: Targeting Matrix Metalloproteinase-9 to Alleviate T Cell Exhaustion and Improve Sepsis Prognosis
    Article Snippet: For real-time reverse transcription polymerase chain reaction (RT-qPCR) assay, total RNA was extracted from PBMCs according to TRIzol reagent (no. 15596026C, Invitrogen) instructions. .. The Reverse Transcriptase Kit (no. RR037A, Takara) was used to synthesize complementary DNA, and the TB Green PCR Kit (no. CN830A, Takara) was used for qPCR. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real-time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real-Time PCR System (Bio-Rad). ..

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure.
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real- time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real- Time PCR System (Bio- Rad). ..

    Article Title: Targeting Matrix Metalloproteinase-9 to Alleviate T Cell Exhaustion and Improve Sepsis Prognosis
    Article Snippet: For real-time reverse transcription polymerase chain reaction (RT-qPCR) assay, total RNA was extracted from PBMCs according to TRIzol reagent (no. 15596026C, Invitrogen) instructions. .. The Reverse Transcriptase Kit (no. RR037A, Takara) was used to synthesize complementary DNA, and the TB Green PCR Kit (no. CN830A, Takara) was used for qPCR. ..

    Polymerase Chain Reaction:

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real-time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real-Time PCR System (Bio-Rad). ..

    Article Title: Enhanced FOS expression improves tumor clearance and resists exhaustion in NR4A3-deficient CAR T cells under chronic antigen exposure.
    Article Snippet: RNA was extracted after CAR T cells were sorted by TRIzol (Invitrogen, catalog no. 15596026). .. The cDNA was synthesized with a reverse transcriptase kit (Takara, catalog no. RR047A), and real- time polymerase chain reaction (PCR) was performed with a SYBR Premix Ex Taq Kit (Takara, catalog no. CN830S) in a CFX96 Touch Real- Time PCR System (Bio- Rad). ..

    Article Title: Targeting Matrix Metalloproteinase-9 to Alleviate T Cell Exhaustion and Improve Sepsis Prognosis
    Article Snippet: For real-time reverse transcription polymerase chain reaction (RT-qPCR) assay, total RNA was extracted from PBMCs according to TRIzol reagent (no. 15596026C, Invitrogen) instructions. .. The Reverse Transcriptase Kit (no. RR037A, Takara) was used to synthesize complementary DNA, and the TB Green PCR Kit (no. CN830A, Takara) was used for qPCR. ..

    Extraction:

    Article Title: Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial Cells
    Article Snippet: .. After chloroform extraction and isopropanol precipitation, RNA was reverse-transcribed into cDNA using a reverse transcriptase kit (Cat. No. #RR036A; Takara Ltd., Otsu, Japan). .. Quantitative PCR was performed using TB Green Premix Ex Taq II (Cat. No. #RR820A; Takara Ltd). on an ABI 7300 realtime PCR system (Applied Biosystems Inc).

    Article Title: Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial Cells
    Article Snippet: .. After chloroform extraction and isopropanol precipitation, RNA was reverse-transcribed into cDNA using a reverse transcriptase kit (Cat. No. #RR036A; Takara Ltd., Otsu, Japan). .. Quantitative PCR was performed using TB Green Premix Ex Taq II (Cat. No. #RR820A; Takara Ltd). on an ABI 7300 real-time PCR system (Applied Biosystems Inc).

    cDNA Synthesis:

    Article Title: Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver disease via GLUD1/α-KG axis
    Article Snippet: Journal Pre-proofs Article Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver dis‐ ease via GLUD1/α-KG axis Mengya Geng, Fanzheng Meng, Hairui Li, Lijie Sun, Xuedan Sun, Yu Ding, Rongdongqing Shi, Zhihua Wang, Yabin Huang, Jizhou Wang, Yao Liu, Jiabei Wang, Peter J.. Little, Suowen Xu, Lianxin Liu, Jianping Weng, Sihui Luo PII: S2095-9273(26)00365-8 DOI: https://doi.org/10.1016/j.scib.2026.04.016 Reference: SCIB 3996 To appear in: Science Bulletin Received Date: 28 October 2025 Revised Date: 24 February 2026 Accepted Date: 3 April 2026 Please cite this article as: M. Geng, F. Meng, H. Li, L. Sun, X.. Sun, Y. Ding, R. Shi, Z. Wang, Y. Huang, J. Wang, Y. Liu, J. Wang, P.J.



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